Brett Savary - Research Professor of Protein and Cell Wall Chemistry

Brett Savary jpg
Phone: 870-680-4792 (Office) 870-680-4793 (Lab)
Address: Arkansas Biosciences Institute I PO Box 639 I State University AR 72467
Email: bsavary@astate.edu

Affiliations:

Arkansas Biosciences Institute at Arkansas State University and the College of Agriculture and Technology

Cluster Identification:
- Plant Interactions with Other Organisms

 

Research Areas/Expertise:
- Plant cell walls: pectin and arabinoxylan chemistry, glycohydrolases
- Enzyme purification and characterization; bioseparations chemistry
- Protein bioproduction in Pichia pastoris
Biomolecule mass spectrometry with MALDI-TOF
- Healthful food and feed products from agricultural and food processing residues (rice bran and sugar beets)
- Hairy-root cultures: root-specific proteomics and bioactive proteins
- Biochemical ecology of white-nose syndrome in bats; Pseudogymnoascus destructans serine proteases

Research Summary | Selected Publications | Lab Members | Key Collaborators | Research Projects | Links

Research Summary:

My research interests cover the production, characterization, and application of plant and microbial glycohydrolases and their application to generate functional polysaccharide-derived products from plant cell walls in food and biofuel processing residues (citrus, rice, and sugar beets).  I engage with multidisciplinary collaborators to generate fundamental knowledge that will enable rational design of polysaccharide-derived bioproducts with improved bioavailability, performance, and functionality for commercial utility and human dietary health.  My laboratory contributes hands-on training and applications development of the MALDI-TOF mass spectrometer acquired through the NSF EPSCoR/AR ASSET Plant-Powered Production Center at ASU.

Selected Publications:

• Zhang, N, M Gonzalez, BJ Savary, J Xu (2015). High-yield secretion of recombinant proteins in tobacco cell culture with HypGlyco technology: process development. Biotechnol J, December 2 (Early View), DOI: 10.1002/biot.201500377.

• Tovar, J, J Xu, BJ Savary (2015). Plant pectin methylesterase treatments dramatically reduce water-binding in sugar beet pulp via improved calcium crosslinking. IN Proceedings of the 38th Biennial Meeting of the American Society of Sugar Beet Technologists (Vol. 38). Clearwater Beach, FL: http://assbt-proceedings.org/ASSBT2015Proceedings/Section%20C/Tovar.pdf

• Zhang, N, J Xu, BJ Savary (2015). Thermostable endo-1,5-α-L-arabinanase for isolating functional oligosaccharides from sugar beet pulp.  IN Proceedings of the 38th Biennial Meeting of the American Society of Sugar Beet Technologists (Vol. 38). Clearwater Beach, FL: http://assbt-proceedings.org/ASSBT2015Proceedings/Section%20C/Zhang.pdf

 Savary, BJ, J Xu, JC Tovar, N Zhang, H Fang (2015). Thermostable pectinase technologies for remodeling sugar beet root cell walls and generating functional products. IN Proceedings of the 38th Biennial Meeting of the American Society of Sugar Beet Technologists (Vol. 38). Clearwater Beach, FL: http://assbt-proceedings.org/ASSBT2015Proceedings/Section%20C/Savary.pdf

• Pannkuk, EL, TS Risch, BJ.Savary (2015).  Isolation and identification of an extracellular subtilisin-like serine protease secreted by the bat pathogen Pseudogymnoascus destructans.  PLOS One 10(3): e0120508.

• Pannkuk, EL, HB Blair, AE Fischer, CL. Gerdes, DF Gilmore, BJ Savary, TS Risch (2014). Triacylglyceride composition and fatty acyl saturation profile of a psychrophilic and psychrotolerant fungal species grown at different temperatures. Fungal Biology 118:792-799.

• Zhang, N, VS Green, X Ge, BJ Savary, J Xu (2014).  Ethanol fermentation of energy beets by self-flocculating and non-flocculating yeasts.  Biores. Technol. 155: 189-197.

Savary BJ, P Vasu, RG Cameron, A Nuñez, TG McCollum (2013). Purification, structural characterization, and cloning of the thermally-tolerant pectin methylesterase from Citrus sinensis. J. Agric. Food Chem. 61: 12711–12719.

• Pannkuk EL, TS Risch, BJ Savary (2013). Profiling the triaclyglyceride contents in bat integumentary lipids by preparative thin layer chromatography coupled to MALDI-TOF mass spectrometry. J. Visual. Exper. 2013 (79): 50757. 

• Kim Y, MAK Williams, AL Galant, GA Luzio, BJ Savary, P Vasu, RG Cameron (2013). Nanostructural modification of a pectin with a papain-stable pectin methylesterase: effects of pH on nanostructure, enzyme mode of action and functionality. Food Hydrocoll. 33(1): 132–141.

• Vasu P, RG Cameron, BJ Savary (2012). Purification and characterization of a papaya (Carica papaya L.) pectin methylesterase isolated from a commercial papain preparation. Food Chemistry 133: 366-372.

Savary BJ, P Vasu (2011). Routine identity confirmation of recombinant proteins by MALDI-TOF mass spectrometry. IN Recombinant Gene Expression, Reviews and Protocols, 3rd edition, A. Lorence (Ed.), New York, NY: Humana Press.

• Vasu P, BJ Savary, S Bauer (2011). Cloning and expression of hemicellulases from Aspergillus nidulans in Pichia pastoris. IN Recombinant Gene Expression, Reviews and Protocols, 3rd edition, A. Lorence (Ed.), New York, NY: Humana Press.

• Cameron RG, GA Luzio, P Vasu, BJ Savary (2011). Enzymatic modification of a model homogalacturonan with the thermally-tolerant pectin methylesterase from Citrus: I. nanostructural characterization, enzyme mode of action and effect of pH. J. Agric. Food Chem. 59: 2717-2724.

Savary BJ, P Vasu, A Nuñez, RG Cameron (2010). Identification of thermolabile pectin methylesterases isolated from sweet orange fruit by peptide mass fingerprinting. J. Agric. Food Chem. 58: 12,462-12,468.

Lab Members:

Thomas (Keat) Teoh, Ph.D., research scientist.
Jose Tovar, doctoral student (advisee), Molecular Biosciences Program.   
Ningning Zhang, doctoral student (co-advisee with Jay Xu) Molecular Biosciences Program.
Megan Cease, undergraduate student and P3 scholar, Biology and Chemistry.

Past P3-Supported Lab Members:

Evan Pannkuk, Ph.D. (graduated 2014; co-advisee with Tom Risch, Biology), Environmental Sciences (2009-2014). [Currently postdoctoral scientist at Georgetown University] 
Brian Mason, undergraduate student and P3 scholar, Biology and Chemistry Honors (2014-2015).
Samantha Davis, undergraduate student and P3 Scholar, Biology (2010-2012.) 
James Miller, undergraduate student and P3 Scholar, Biology and Chemistry Honors (2009-2011).
Prasanna Vasu, Ph.D., research assistant professor (2007-2011). [Currently research scientist at CFTRI, Mysore India]

Key Collaborators

Dr. Jianfeng (Jay) Xu, Biochemical Engineering | Arkansas State University, Jonesboro, AR
Dr. Fabricio Medina-Bolivar, Plant Metabolic Engineering | Arkansas State University, Jonesboro, AR
Dr. Shiguang Yu, Immunology | Arkansas State University, Jonesboro, AR
Dr. Sun-Ok Lee, Nutrition and Food Science | University of Arkansas, Fayetteville, AR
Dr. Ya-Jane Wang, Carbohydrate Chemistry | University of Arkansas, Fayetteville, AR
Dr. Randy Cameron, Research Biologist | USDA Agricultural Research Service, Ft. Pierce, FL
Dr. Ming Chen, Research Chemist | USDA Agricultural Research Service, Stuttgart, AR
Dr. Prasanna Vasu, Bioanalytical Chemistry | Central Food Technological Research Institute, Mysore, India

Research Projects

• Establishing the function and availability of bioactive components from whole-grain rice varieties for colon-specific health benefits. (USDA-NIFA AFRI)
• Separations chemistry to isolate and analyze feruloylated arabinoxylan oligosaccharides generated from rice bran fiber.  (AR ASSET/NSF EPSCoR P3 Center and A-State ABI)
• Biochemical ecology of white-nose syndrome in bats – identification of biomolecules involved in host-pathogen recognition and pathogen establishment. (USFWL/AGFC-SWG)
• Bioproduction of thermostable enzymes and their application to reduce energy inputs in sugar beet processing. (Beet Sugar Development Foundation and DOE)
• Pectin methylesterases – structure, function, and application to food hydrocolloid systems and biomass processing. (A-State ABI and USDA-CSREES-NRI)

Links

P3 YouTube video: Get to know ASU-J Doctoral Student Researcher Jose Tovar
http://www.youtube.com/watch?v=XItGAwxXrvU

P3 YouTube video: AR P3 Center highlights ASU-J doctoral student researcher Ningning Zhang
http://www.youtube.com/watch?v=KE2Gm95Ia2o

JoVE publication video: Evan Pannkuk
http://www.jove.com/video/50757/profiling-triacylglyceride-contents-bat-integumentary-lipids

Biobased Solutions in the College of Agriculture & Technology at Arkansas State University-Jonesboro
http://www.biobasedsolutions.org/#!home-page

A-State Arkansas Biosciences Institute faculty page
http://www.astate.edu/a/abi/faculty-staff/people-details.dot?pid=c9059b80-00dd-4245-87da-0333ac883bde