Fabricio Medina-Bolivar - Associate Professor

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Phone: 870-680-4319 (Office)
Address: P.O. Box 639 I State University AR 72467
Email: fmedinabolivar@astate.edu


ASU: Biology and Arkansas Biosciences Institute

Cluster Identification:
- Comparative Metabolomics, Genomics and Metabolic Engineering Cluster
- Plant Interactions with Other Organisms Cluster

Research Areas/Expertise:
- Antioxidants
- Hairy Root Cultures
- Metabolic Engineering
- Plant Secondary Metabolism
- Phytochemicals & Human Health
- Phytochemical Analysis: HPTLC, HPLC, LC-MS
- Plant Tissue Culture
- Transgenic Plants

Research Summary | Selected Publications | Lab Members | Key Collaborators | Research Projects | Links

Research Summary:

The Medina-Bolivar laboratory focuses on elucidating the mechanisms that control the biosynthesis of selected plant natural products of importance to human health. We utilize hairy root cultures as sustainable bioproduction systems and elicitation and metabolic engineering strategies to increase the levels of these biologically active phytochemicals.  Our areas of study center on the biosynthesis and bioproduction of stilbenoids, flavonoids and alkaloids.

Selected Publications:

Marsh Z, Yang T, Nopo-Olazabal L, Wu S, Ingle T, Joshee N, Medina-Bolivar F. 2014. Effect of light, methyl jasmonate and cyclodextrin on production of polyphenolic compounds in hairy root cultures of Scutellaria Iaterifora. Phytochemistry 107:50-60. 

Zhai B, Clark J, Ling T, Connelly M, Medina-Bolivar F, Rivas F. 2014. Antimalarial evaluation of the chemical constituents of hairy root culture of Bixa orellana. Molecules. 19(1):756-766.

Nopo-Olazabal C, Nopo-Olazabal L, Medina-Bolivar F. 2014. Differential induction of antioxidant stilbenoids in hairy roots of Vitis rotundifolia treated with methyl jasmonate and hydrogen peroxide. Plant Physiology and Biochemistry. 74:50-69.

Nopo-Olazabal C, Hubstenberger J, Nopo-Olazabal L, Medina-Bolivar F. 2013. Antioxidant activity of selected stilbenoids and their bioproduction in hairy root cultures of muscadine grape (Vitis rotundifolia Michx.). Journal of Agricultureal and Food Chemistry. 61(48):11744-11758

Joshee N, Tascan A, Medina-Bolivar F, Parajuli P, Rimando AM, Shannon DA, Adelberg JW. 2013. Scutellaria: Biotechnology, Phytochemistry and its Potential as a Commercial Medicinal Crop. In: Biotechnology for Medicinal Plants: Micropropagation and Improvement, Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer-Verlag, Heidelberg Germany. pp. 69-99.

Prabhu P, Medina-BolivarF, Condori J, Sivakumar G, Srivatsan M. 2012. Neuroprotective effect of peanut hairy root extract against oxidative stress in PC12 derived neurons. Journal of Medicinally Active Plants. 1(4):125-133.

Brents LK, Medina-Bolivar F (*), Seely KA, Nair V, Bratton SM, Gallus-Zawada A, Nopo Olazabal L, Patel RY, Liu H, Doerksen RJ, Prather P, Radominska-Pandya A. 2012. Natural prenylated reseratrol analogs, arachidin-1 and -3, demonstrate an improved glucuronidation profile and have affinity for cannablnold receptors. Xenobiotica. 42:139-156. [*First two authors share first authorship]

Nopo L, Woffenden B, Reed D, Buswell S, Zhang C, Medina-Bolivar F. 2012. Super-promoter: TEV, a powerful gene expression system for tobacco hairy roots.  (In: Recombinant Gene Expression: Reviews and Protocols, Third Edition, A. Lorence (Ed.), Springer). Methods in Molecular Biology. 824:501-526.

Condori J, Nopo-Olazabal C, Medrano G, Medina-Bolivar F. 2011. Selection of reference genes for qPCR in hairy roots cultures of peanut. BMC Research Notes. 4:392

Sivakumar G, Medina-Bolivar F, Lay J, Dolan MC, Condori J, Wright SM, Baque MDA, Lee E-J, Paek KY.  2011. Bioprocess and bioreactor: Next generation technology for production of potential plant-based antidiabetic and antioxidant molecules.  Current Medicinal Chemistry. 18:79-90.

Abbott J, Medina-Bolivar F, Martin E, Engelberth AS, Villagarcia H, Clausen EC, Carrier DC. 2010. Purification of resveratrol, arachidin-1 and arachidin-3 from hairy root culture of peanut (Arachis hypogaea) and determination of their antioxidant activity and cytotoxicity. Biotechnology Progree. 26(5):1344-1351 

Condori J, Sivakumar S, Hubstenberger J, Dolan MC, Sobolev VS, Medina-Bolivar F. 2010. Induced biosynthesis of reveratrol and the prenylated stillbenolds arachihinn-1 and archidin-3 in hairy root cultures of peanut: Effects of culture medium and growth stage. Plant Physology and Biochemstry. 48:310-318.  

Condori J, Medrano G, Sivakumar G, Nair V, Cramer C, Medina-Bolivar F. 2009. Functional characterization of a stilbene synthase gene using a transient expression in planta. Plant Cell Reports 28:589-599.

Medina-Bolivar F, Condori J, Rimando A, Hubstenberger J, Shelton K, Bennett S, Dolan M. 2007. Production and secretion of resveratrol in hairy root cultures of peanut. Phytochemistry. 68:1992-2003.

Lab Members:

Current Members

Fabricio Medina-Bolivar, Ph.D.
Associate Professor of Metabolic Engineering
Department of Biological Sciences
Arkansas Biosciences Institute
fmedinabolivar@astate.edu; (870) 680-4319
Luis Nopo-Olazabal, Ph.D.
Research Associate
lnopo@astate.edu; (870)680-4320
Tianhong Yang, M.S.
Ph.D. student
Molecular Biosciences Graduate Program
tianhong.yang@smail.astate.edu; (870) 680-4320
  Lingling Fang
Research Assistant
linglingfang.0131@gmail.com; (870) 680-4320
  Johnvonta Brooks
Research Assistant
johnvontabrooks@gmail.com; (870) 680-4320
  Sarah Collier
Undergraduate Student
sarah.griffin@smail.astate.edu; (870) 680-4320
  Jarrod Creameans
Undergraduate Student
jarrod.creameans@smail.astate.edu; (870) 680-4320
  Tyler Knapp
Honors Undergraduate Student
Biology and Chemistry
tyler.knapp@smail.astate.edu; (870) 680-4320
  Nawja Lee
Undergraduate Student
nawja.lee@smail.astate.edu; (870) 680-4320
  Christopher Aaron Tollett
Honors Undergraduate Student
Biology and Chemistry
christop.tollett@smail.astate.edu; (870) 680-4320

Past Members

Dr. Cesar Nopo-Olazabal
Graduated: Summer 2013 (Ph.D. Molecular Biosciences)
Now: Research Scientist; Altria, Richmond, Virginia                                                   
Zachary Marsh
Graduated: Spring 2013 (Honors, Biology)
Now: Master's student, Public Health in Epidemiology; Emory University, Atlanta, Georgia
  Bo Zhai
Graduated: Spring 2012 (M.S. Biotechnology)
Now: Ph.D. student, Veterinary Biomedical Sciences; Oklahoma State University, Stillwater, Oklahoma
  Carlos Balmaceda
Graduated: Fall 2011
Now: Master's student, Crop Biotechnology and Entrepreneurship; The University of Nottingham, England
  Anandeep Patel (M.S. Biotechnology)
Graduated: Spring 2013
Now: Research Technician; Texas Medical Center, Houston, TX

For detailed information of former laboratory members, please visit: http://www.clt.astate.edu/fmedinabolivar

Key Collaborators

Dr. Nirmal Joshee, Fort Valley State University, Fort Valley, Georgia 

Dr. Keithanne Mockaitis, Associate Scientist, Indiana University, Bloomington, Indiana

Dr. Anna Radominska-Pandya, Professor, University of Arkansas for Medical Sciences, Little Rock 

Dr. Agnes Rimando, USDA, Natural Products Utilization Research Unit, Oxford, Mississippi

Dr. Malathi Srivatsan, Profesor, Arkansas State University, Jonesboro, Arkansas

Dr. Brett Savary, Professor, Arkansas State University, Jonesboro, Arkansas

Dr. Victor Sobolev, USDA, National Peanut Research Laboratory, Dawson, Georgia

Research Projects

-Bioproduction of resveratrol and related stilbenoids in hairy root cultures of peanut and muscadine grape

-Elucidation of stilbenoid biosynthesis in peanut

-Biological activity of natural prenylated resveratrol analogs

-Metabolic engineering of flavonoid biosynthesis in Scutellaria lateriflora (American skullcap) by expression of selected transcription factors in hairy roots

-Bioproduction of anticancer alkaloids in tissue cultures of monocot species


Medina-Bolivar laboratory at Arkansas State University (hosted by the Medina-Bolivar laboratory): http://www.clt.astate.edu/fmedinabolivar/


Dr. Medina-Bolivar was the Program Chair and Host of the 3rd Annual Conference of American Council for Medicinally Active Plants (ACMAP). For a highlight of the conference check the following video: http://www.youtube.com/watch?v=9VEwO6fYCgQ&feature=plcp

American Council for Medicinally Active Plants: http://www.acmap.org

Medina-Bolivar's P3 YouTube Video: http://youtu.be/LNs5L_q6e84

P3 Stilbenoid Symphony YouTube Video: http://www.youtube.com/watch?v=sm6_p6DBJRg